Bacterial Transformation - Describe how you will be able to tell if the bacterial transformation experiment worked.
- During this lab, two genes were put into bacteria. Describe what each of these genes do.
- State the function of the "heat shock".
Suppose that the following kinds of plates were prepared: a) LB b) LB/amp c) LB/amp/arab. The next four questions ask what plates bacteria will grow on. To answer the questions, be sure to list all of the plates which will produce growth. - If transformed bacteria were added to each of the plates, which plate(s) (a, b, and/or c) should the transformed bacteria grow on? Why? (You must explain why to receive points for this question.)
- If untransformed bacteria were added to each of the plates, which plate(s) (a, b, and/or c) should untransformed bacteria grow on? Why? (You must explain why to receive points for this question.)
- Which plate(s) (a, b, and/or c) should contain bacteria that glow under UV light? Why? (You must explain why to receive points for this question.)
- Which plate(s) (a, b, and/or c) would be used to select for bacteria that were ampicillin resistant? Why? (You must explain why to receive points for this question.)
- Explain why bacteria that glow under UV light should also be ampicillin resistant.
- The DNA that contained the genes that were put in the bacteria was
a. a virus b. a ribosome c. a plasmid d. an enzyme e. unstable - Explain how the arabinose operon works.
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