Bacterial Transformation

1. Describe how you will be able to tell if the bacterial transformation experiment worked.
2. During this lab, two genes were put into bacteria. Describe what each of these genes do.
3. State the function of the "heat shock".

   Suppose that the following kinds of plates were prepared:
   a) LB
   b) LB/amp
   c) LB/amp/arab.
   The next four questions ask what plates bacteria will grow on. To answer the questions, be sure to list all of the plates which will produce growth.

    

4. If transformed bacteria were added to each of the plates, which plate(s) (a, b, and/or c) should the transformed bacteria grow on? Why? (You must explain why to receive points for this question.)
5. If untransformed bacteria were added to each of the plates, which plate(s) (a, b, and/or c) should untransformed bacteria grow on? Why? (You must explain why to receive points for this question.)
6. Which plate(s) (a, b, and/or c) should contain bacteria that glow under UV light? Why? (You must explain why to receive points for this question.)
7. Which plate(s) (a, b, and/or c) would be used to select for bacteria that were ampicillin resistant? Why? (You must explain why to receive points for this question.)
8. Explain why bacteria that glow under UV light should also be ampicillin resistant.
9. The DNA that contained the genes that were put in the bacteria was
   a. a virus b. a ribosome c. a plasmid d. an enzyme e. unstable
10. Explain how the arabinose operon works.