Microscopy

1. Suppose that a person places a ruler under a microscope and measures the field of view to be 3 mm (3000 micrometers) using the 4 X objective. How big will the field of view be if she switches to a 43 X objective? Your answer should be in micrometers.

2. Which power has a greater depth of focus (depth of field)?

   a) low power     b) medium power     c) high power

3. The eyepiece of a microscope is 10X.  If the objective lens also has a magnification of 43X, what is the total magnification?

4. Suppose that you are instructed to view an object under high power (not oil immersion).  Describe the steps used to find and focus the object.

5. View the microscope slide of Paramecium under high power.

6. Make a wet mount of cheek cells

7. State the advantage of using a dissecting microscope over a compound light microscope.

8. Describe the function of the parts of the microscope in the photograph. Notice that this question does not ask you to name the part.