Ecdysozoans - Roundworms, Arthropods

Roundworms

Read "Roundworms" in the notes.

Ascaris Dissection

1. Obtain a female Ascaris and place it on a dissecting pan. The posterior end is curved in males and straight in females.

2. Make a shallow longitudinal cut the length of the worm and pin it open as illustrated below.

3. Identify the gut, ovary, oviduct, uterus, and vagina.

Trichinella

View a prepared slide of Trichinella in the muscle tissue of a host organism.

Trichinella spiralis encysted
in muscle tissue of a host
X 40

Vinegar eels

1. Observe vinegar eels using scanning (40X) or low (100X magnification. Observe the gut within the pseudocoelom. The tube-within-a-tube body plan of these pseudocoelomate animals can be seen in the photograph of a vinegar eel (below).

Arthropods

Exoskeleton

Arthropods have an exoskeleton that cannot enlarge as the animal grows. As a result, it must be shed from time to time to allow growth. Young crayfish have been placed in an aquarium so that we can observe their molting. Sometime over the next several weeks, you should be able to find exoskeletons on the bottom of the tank. The exoskeleton from a tarantula (an arachnid) is on display.

Life Cycle

The young of some arthropods look like the adults. The change from young to adult that these species undergo is called incomplete metamorphosis.

In many species, the egg hatches to produce a larva (pl. larvae) that does not look like the adult. At some point in the maturation process, the larva will produce a pupa (pl. pupae). In this phase its tissues will become reorganized into the adult form. This type of development is called complete metamorphosis.

Observe a culture of fruit flies (Drosophila) and observe the eggs, larvae, pupae, and adults.

Cheliceriformes

Read the following topics in your lecture notes booklet:

Overview of Arthropods (phylum: Arthropoda)

Cheliceraformes

The first pair of appendages of the members of this phylum are pincerlike or fanglike mouthparts called chelicerae. Observe these mouthparts in the tarantula on display.

Spiders and relatives (Class Arachnida)

Scorpion
Tick X 40
Mite X 40

Horseshoe Crabs (Class Merostomata)

Horshoe crab dorsal surface
Horshoe crab ventral surface

Crustaceans (subphylum Crustacea) - Lobsters and relatives

Read the following topic in your lecture notes booklet: Crustaceans (subphylum Crustacea) - Lobsters and relatives

Decapods

Crab

Crayfish Dissection

Obtain a crayfish and identify the structures shown in the photograph below. You should familiarize yourself with the names of the external structures shown in the photograph below.

The photographs below show the ventral surface of male and female crayfish. Click on the photographs to view them. Identify the sex of your crayfish. Find somebody in class that has the opposite sex and view the ventral surface of their crayfish. Notice that crayfish have five pairs of swimmerets. In males, the anterior two pairs are large and less flexible than those of females. They are used to transfer sperm to the female.

Find the seminal receptacles located between the third and fourth walking legs. These structures function to receive sperm from the male during mating.

Observe the sperm duct in males located at the base of the fifth walking leg.

Begin your dissection by cutting along the midline of the carapace from the posterior edge to an area just behind the two eyes (see the photographs below). Next, cut laterally just behind the eye until you reach the ventral edge. Carefully remove the piece of carapace that you have cut to expose the gills underneath. As you peel this piece of carapace away, it may be necessary to reach underneath with the point of a scissors or needle to brush away and detach any tissue from the interior of the animal that is attached to the carapace.

Click on the photographs to view enlargements.

Your crayfish should look like the first photograph below. Next, remove the carapace on the other side.

Using a needle, carefully separate the first row of gills and notice that there is another row underneath. Remove one of the legs and observe how the gills are attached to the walking legs.

The first two photographs below show the gills on the left side of a crayfish. The third photograph is a crayfish with the entire carapace removed. The first row of gills on each side has been moved aside to expose a row underneath it.

Below: Crayfish leg showing gill attachment

Brush all of the gills to the side and then carefully cut the membrane (epidermis) that covers the internal organs.

The heart is a small diamond-shaped structure located below where the posterior edge of the carapace was. It may be difficult to see the blood vessels attached to the heart. Openings (ostia) in the side of the heart should be visible. These allow blood to enter the circulatory system.

Large white digestive glands can be seen on each side of the stomach. They produce digestive enzymes.

Remove the digestive glands and then carefully remove the stomach. Notice how the stomach is attached to the mouth. Cut open the stomach and observe the tooth-like structures at the anterior end for grinding food. This is called the gastric mill.

The green glands are positioned ventrally near the anterior end of the body cavity. They are spherical and will appear to be embedded within the surface. Their function is excretion. Find the openings for the green glands on the outer surface near the base of each antenna.

Find the brain near the anterior end and the two ventral nerve cords attached to the brain.

Below:

Crayfish ventral surface
Crayfish viewed from above. The stomach has been removed revealing the nerve cords and green glands underneath.
Crayfish viewed from above looking into the head region. The stomach has been removed. The brain can be seen.