LabPrepSnyderBio1

Clinton Community College, Plattsburgh, New York 12901

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Science Laboratory Prep Schedule

Bio I

Michele Snyder

Lab Title	Prep Materials
Safety, Metric Measurement and Intro to Microscopy	Part I - Various Slides - slides and coverslips - Yogurt part II: - Onion - Elodea - Potato - IKI ( iodine solution) - Toothpicks ( wrapped) - Methylene Blue stain - Baker's Yeast - Hay Infusion - Please click to make changes**
Microscopy and Scientific Method Exp. Design w/ Euglena	- Euglena Media ( sterilized ) - Live Euglena ( live delivery over weeks of labs ) - Orb Shaker - Elodea - Please click to make changes**
Macromolecules	1% Glucose 1% Sucrose 1-2% Starch 1% Albumin, 1-2% Pepsin, Benedict's solution IKI solution Vegetable Oil 0.2% HCl Biuret ( 3% CuSO4; 10% KOH) Other materials: Onion, Potato, Brown Paper squares Rubber stoppers - Please click to make changes**
Cells and Transport	- 10% Starch Soln - 10% NaCl Soln - 10% Glucose Soln - 10% Albumin Soln - Elodea - Please click to make changes**
Enzymes: Lactase - lactose intolerance	Lactaid Fast Act Grease pencils Glucose Test Strips 100/ lab Week 1 Milks: - Cow, Soy Rice ( Unk 1, Unk 2, Unk 3) Mortar and Pestle ( 4 min ) Short Tubes (to get forceps into) Conical Centrifuge Tubes large, plastic 1%Glucose Soln - as a positive ICE Week 2 Milk - Cow ( 2x 250ml ) labeled as Cow pH solns: - pH 2,4,7,10 and 12 ( made with HCl and NaOH ) Mortar and Pestle ( 4 min ) Short Tubes (to get forceps into) Conical Centrifuge Tubes large, plastic ICE - Please click to make changes**
Photosynthesis prt I	Spinach in Bag Phosphate Buffer (per liter ) - 8.70g K-phosphate diabasic - 6.80g K-phosphate monobasic 80% Acetone aloquats (10ml) - Please click to make changes**
Photosynthesis prt II	Elodea pH buffers 4 and 7 for calibration ( optional ) - Please click to make changes**
Mitosis and Cell Cycle	- Please click to make changes**
Mendelian Genetics	- Please click to make changes**
Mendelian Genetics Yeast	Yeast strains (9): - HA2 - HBT - HAO - HAR - HAT - HART - HBO - HBR - HBRT Media: - YED plates 30 / class - MVA plates 5 / class - MV plates 5 / class - Please click to make changes**
DNA Finger Printing / Blood Typing	Edvotek Kit # 109: - make Buffer dilution - pour gels - set up E-phoresis app. Blood Typing Kit ( synth. ABO ) - Please click to make changes**
Bact Trans	BioRad Kit P-Glo Trans - make Plates 1 LB/ 2 LB-amp/ 1 LB-amp-arabinose per group - 24 hours ahead - grow E. coli - aliquot broth and tran. soln - check water bath temp - ice on day of lab - Please click to make changes**
Transcription & Translation	- Please click to make changes**
Cancer Gene Detect. I	Gels for Electrophoresis - Please click to make changes**
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Osmoregulation	- Vegetable cubes - 1%, 5%, 10% KMnO4 solutions - Vernier Calipers - Stopwatch or Clock - 10% Starch Soln - 10% NaCl Soln - 10% Glucose Soln - 10% Albumin Soln - 0.9% NaCl Soln - 0.1% NaCl Soln - 5% glucose Soln - 1% glucose Soln - Please click to make changes**
Enzymes: Catalase	Week 1: The study of ENZYME CONCENTRATION Solutions and Other Consumables made ahead of time: Potato Extract (contains the enzyme of study, catalase). Make just before the lab. Combine 250g of potato with 100 mL of phosphate buffer in blender. Filter through a layer of cheese cloth and wire strainer. Each group will need 10mL aliquoted for their own use. (10mL/group x ~6 groups/lab) 0.1M phosphate buffer –Each group will need 10mL buffer aliquoted for their own use. Make anytime and aliquot into 10mL tubes. (10mL/ group x ~6 groups/lab) 1% Hydrogen peroxide (80mL/group x ~6 groups/lab = 480mL) Bucket of Ice Week 2: The study of SUBSTRATE CONCENTRATION Solutions and Other Consumables made ahead of time: Potato Extract (contains the enzyme of study, catalase). Make just before the lab. Combine 250g of potato with 100 mL of phosphate buffer in blender. Filter through a layer of cheese cloth and wire strainer. Each group will need 10mL aliquoted for their own use. (10mL/group x ~6 groups/lab) .1M phosphate buffer –Each group will need 10mL buffer aliquoted for their own use. Make anytime and aliquot into 10mL tubes. (10mL/ group x ~6 groups/lab) Various H₂O₂ Concentrations: Made from 3% hydrogen peroxide for a total volume of 500mL for each of the concentrations below- 0.3%, 0.6%, 0.8%, 1.0%, 1.5%, and 2.0% Bucket of Ice Forceps Paper filter disks 6 glass vials 10mL pipette tip with ratchet dispenser 10 ml of 100% potato extract 10 mL of 0.1M phosphate buffer ( 20-200 uL micropipettors and tips 200-1000 uL micropipettors and tips 8 Microcentrifuge tubes Sponge to hold microcentrifuge tubes Black marker Stop watch Ice/plastic beakers for ice 10mL of Potato extract –need holder for the tube 10mL of phosphate buffer- need holder for the tube (100mM Phosphate Buffer ( 1 liter - 8.70 K2HPO4 dibasic; 6.80 KH2PO4 )) - Please click to make changes**

Contact info:

Room 221 Stafford Center for Art, Science and Technology

Clinton Community College

136 Clinton Pt. Dr.

Plattsburgh, NY 12901

518-562-4337

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