ML HOME | email me Michael Lawliss | CLINTON
Science Laboratory Prep Schedule
Human Bio
Sarah McCoy
| Jan 2010 | ||||
| Monday | Tuesday | Wednesday | Thursday | Friday |
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| 11 |
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13 |
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| 18 |
19 |
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| 25 |
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27 Safety and Metric |
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29 |
| Feb 2010 | ||||
| Monday | Tuesday | Wednesday | Thursday | Friday |
| 1 |
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3 Microscopy and Cell Structure |
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| 8 |
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10 Microscopy |
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| 15 |
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17 *** |
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| 22 |
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24 Transport |
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| Mar 2010 | ||||
| Monday | Tuesday | Wednesday | Thursday | Friday |
| 1 |
2 |
3 Enzymes I |
4 |
5 |
| 8 |
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10 Enzymes II |
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12 |
| 15______________ |
SPRING BREAK |
19______________ |
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| 22 |
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24 Cancer Gene |
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| 29 |
30 |
31 **** |
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| Apr 2010 | ||||
| Monday | Tuesday | Wednesday | Thursday | Friday |
| 1 NO CLASSES |
2 NO CLASSES |
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| 5 |
6 |
7
Histology |
8 |
9 |
| 12 |
13 |
14 Blood Typing/ Heart dissection |
15 |
16 |
| 19 |
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21 Dissections ORGANS |
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| 26 |
27 |
28 Fetal Pig |
29 | 30 |
| May 2010 | ||||
| Monday | Tuesday | Wednesday | Thursday | Friday |
| 3 |
4 |
5 Pig part 2 |
6 |
7 |
| 10 |
11 |
12 **** |
13 |
14 |
| 17 |
18 |
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|
Lab Title |
Prep Materials |
| Safety, Metric Measurement and Intro to Microscopy |
Part I - Various Slides - slides and coverslips - Yogurt part II: - Onion - Elodea - Potato - IKI ( iodine solution) - Toothpicks ( wrapped) - Methylene Blue stain - Baker's Yeast - Hay Infusion ReqFormSM |
| Microscopy and Scientific Method Exp. Design w/ Euglena |
- Euglena Media ( sterilized ) - Live Euglena ( live delivery over weeks of labs ) - Orb Shaker - Elode - yogurt - slides - cover-slips - prepared slides with bacteria ReqFormSM |
| Macromolecules |
1% Glucose 1% Sucrose 1-2% Starch 1% Albumin, 1-2% Pepsin, Benedict's solution IKI solution Vegetable Oil 0.2% HCl Biuret ( 3% CuSO4; 10% KOH) Other materials: Onion, Potato, Brown Paper squares Rubber stoppers ReqFormSM |
| Cells and Transport |
- 10% Starch Soln - 10% NaCl Soln - 10% Glucose Soln - 10% Albumin Soln - Dialysis tubing - Silver Nitrate Soln - Elodea ReqFormSM |
| Enzymes: Lactase - Lactose Intolerance |
Lactaid Fast Act Milks: - Cow, Soy Rice Glucose Test Strips 100/ lab pH solns: - pH 2,4,7,10 and 12 ( made with HCl and NaOH ) Short test tubes ( screw top culture tubes ) - racks to fit them Plastic 1L beakers Mortar and Pestle ( 4 min ) Conical centrifuge tubes ( plastic) Water bath ( boiling ) ICE ReqFormSM |
| Photosynthesis prt I |
Spinach in Bag Phosphate Buffer (per liter ) - 8.70g K-phosphate diabasic - 6.80g K-phosphate monobasic 80% Acetone aloquats (10ml) ReqFormSM |
| Photosynthesis prt II |
Elodea pH buffers 4 and 7 for calibration ( optional ) ReqFormSM |
| Mitosis and Cell Cycle | ReqFormSM |
| Mendelian Genetics | ReqFormSM |
| Mendelian Genetics Yeast |
Yeast strains (9): - HA2 - HBT - HAO - HAR - HAT - HART - HBO - HBR - HBRT Media: - YED plates 30 / class - MVA plates 5 / class - MV plates 5 / class ReqFormSM |
| DNA Finger Printing / Blood Typing |
Edvotek Kit # 109: - make Buffer dilution - pour gels - set up E-phoresis app. Blood Typing Kit ( synth. ABO ) ReqFormSM |
| Bact Trans |
BioRad Kit P-Glo Trans - make Plates 1 LB/ 2 LB-amp/ 1 LB-amp-arabinose per group - 24 hours ahead - grow E. coli - aliquot broth and tran. soln - check water bath temp - ice on day of lab ReqFormSM |
| Transcription & Translation | ReqFormSM |
| Cancer Gene Detect. I | Gels for Electrophoresis |
| ReqFormSM | |
| ReqFormSM | |
| ReqFormSM | |
| Osmoregulation |
- Vegetable cubes - 1%, 5%, 10% KMnO4 solutions - Vernier Calipers - Stopwatch or Clock - 10% Starch Soln - 10% NaCl Soln - 10% Glucose Soln - 10% Albumin Soln - 0.9% NaCl Soln - 0.1% NaCl Soln - 5% glucose Soln - 1% glucose Soln -ReqFormSM |
| Enzymes: Catalase |
100mM Phosphate Buffer ( 1 liter - 8.70 K2HPO4 dibasic; 6.80 KH2PO4 ) - filter paper discs ( many ) - Stop Watches - H2O2 ( 0.3%, 0.6%, 0.8%, 1.0%, 1.5%, 2.0%) - glass vials (approx 50 ml) - 10 ml dispenser - Blender - Ice - Aluminum Foil - Potatoes ReqFormSM |
|
Lab Title |
Prep Materials |
| Metric Meas. | From materials in LAB already. ReqFormSM |
| Stats and Graphing | From materials in LAB already. ReqFormSM |
| Statistical Analysis | From materials in LAB already. ReqFormSM |
| What is Science | From materials in LAB already. ReqFormSM |
| Microscopy | - Toothpicks - Methylene Blue - Onion - 'e' and threads slides ReqFormSM |
| Biochemistry | SOLUTIONS: 1% Glucose 1% Sucrose 1-2% Starch 1% Albumin, 1-2% Pepsin, Benedict's solution IKI solution Vegetable Oil 0.2% HCl Biuret ( 3% CuSO4; 10% KOH) Other materials: Onion, Potato, Brown Paper squares Rubber stoppers ReqFormSM |
| Cells | Living: amoeba; elodea, potato SOLUTIONS: Methylene Blue Potassium permanganate crystals 50% Molasses 10% NaCl; 0.9% NaCl Sheep's Blood Other materials: Clear Gelatin Tubes w/ dye Dialysis Tubing; w/ clamps ReqFormSM |
| Enzymes | SOLUTIONS: Milk 2% Renin ( 0.5% ) split into 3 equal flasks Hydrogen Peroxide 6M HCl ; 5M NaOH Urea 5% solution Urease <1% solution Other materials: potato; sand; spatulas pH paper (1-12) ReqFormSM |
| Photosynthesis | Living: Elodea SOLUTIONS: Spinach Extract Chromatography solvent ( 90% Petroleum Ether/10% Acetone) Other materials: Lamps ( 100W bulbs ) 2-liter Beakers pH Probes; calib. buffers ( pH 4 and pH 10 ) Chromatography materials - cork stoppers w/ paper clips - large test tubes - capillary tubes or Pasteur pipettes - chromatography paper ReqFormSM |
| Cell. Resp. | Living: Yeast suspension germinated bean seeds dry bean seeds SOLUTIONS: 2% Glucose 2% Sucrose 2% Fructose 2% Starch dye solution KOH 15% Other materials: Cotton spatulas ReqFormSM |
| Mitosis & Meiosis | N/A ReqFormSM |
| Pedigree Analysis | N/A ReqFormSM |
| Nondisjunction | N/A ReqFormSM |
| Bacterial Trans | Prep as per Kit instructions - ice - micropipetters optional ReqFormSM |
| DNA Fingerprinting | Prep as per Kit instructions - micropipetters - set up Electrophoresis Equipment ReqFormSM |
| ReqFormSM |
| Room 221 Stafford Center for Art, Science and Technology |
| Clinton Community College |
| 136 Clinton Pt. Dr. |
| Plattsburgh, NY 12901 |
| 518-562-4337 |
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