Name ________________________________________________ Section (circle one):  1    2    3

Lab Partner(s)__________________________________________________________________

Enter “none” on the line above if you worked alone.

Data Sheet

Part A - Effect of Temperature on Enzyme Activity

A1.  Two hypotheses (one should address the effect of temperature, the other should address the effect of denaturing the enzyme):

Table 1- Observations on the effect of temperature on the rate of an enzyme reaction in this table.

A9.  Do your results support either of your hypotheses? Explain.

Part B - Effect of pH on enzyme activity

B1.  Hypothesis or hypotheses:

Table 2. Observations on the effect of an acid and a base on enzyme activity.

B11.  At what pH did catalase function best in this experiment?

B12.  Do your results support your hypothesis? Explain.

B13.  In part A of this lab, you worked with rennin, an enzyme found in the stomach of mammals. The pH of the stomach is normally about 2.0. At what pH do you predict rennin works best?

B14.  What can you say about pH and enzyme functioning? Is there a single pH that enzymes function best at or does it depend on the enzyme?

Part C - Effect of enzyme concentration on rate of reaction

C1.  Hypotheses:

Table 3. The number of drops of urease added to each tube and the time it took to produce a reaction.

C8.  Did using less enzyme produce a reaction?

C9.  What was the effect of using less enzyme in your experiment? If your experiment did not work as expected, what should have happened?

C10.  In general, what happens to the rate of reaction as the amount of enzyme is decreased?

C11.  Do your results support your hypothesis? Explain.