DNA Fingerprinting

1. Based on the results below, the DNA of which suspect was found at the crime scene? a. Suspect 1 b. Suspect 2 c. Both d. Neither

   Lane 1: DNA sample from crime scene cut with Enzyme 1 
   Lane 2: DNA sample from crime scene cut with Enzyme 2 
   Lane 3: DNA sample from Suspect 1 cut with Enzyme 1 
   Lane 4: DNA sample from Suspect 1 cut with Enzyme 2 
   Lane 5: DNA sample from Suspect 2 cut with Enzyme 1 
   Lane 6: DNA sample from Suspect 2 cut with Enzyme 2

   

2. In electrophoresis, the longest fragments of DNA a. Become attached to the dye b. Move the fastest c. Produce double bands d. Move the slowest
3. DNA used in DNA fingerprinting (RFLP analysis) a. Produces bands on the gel only if the DNA is mixed with mRNA. b. Contains only one gene. c. Has been cut with restriction enzymes before placing it on the gel. d. Is used whole and uncut. e. Is cut by the electric current after it is placed on the gel.
4. The purpose of staining is to a. Allow the identification of individual chromosomes. b. Allow the DNA to be seen. c. Enable the chromosomes to migrate faster in the electric current. d. Neutralize the negative charge normally found on DNA. e. Both c and d.